Document

We consent to the incorporation by reference in the Registration Statements (Nos. 333-232705, 333-207659 and 333-206473) on Form S-3 and Registration Statements (Nos. 333-217718 and 333-149914) on Form S-8 of MidWestOne Financial Group, Inc. and subsidiaries, of our reports dated March 11, 2021, relating to the consolidated financial statements, and the effectiveness of internal control over financial reporting of MidWestOne Financial Group, Inc. and subsidiaries, appearing in the Annual Report on Form 10-K of MidWestOne Financial Group, Inc. and subsidiaries for the year ended December 31, 2020.