EXHIBIT 21

PERDOCEO EDUCATION CORPORATION

List of Subsidiaries

as of December 31, 2024

Subsidiary

State of Organization

AIU Online, LLC

DE

American InterContinental University System, Inc.

GA

Career Education Student Finance LLC

DE

CEC Educational Services, LLC

IL

CEC Employee Group, LLC

DE

CEC Real Estate Holding, Inc.

DE

Colorado Tech, Inc.

DE

Colorado Technical University, Inc.

CO

Hippo Education, LLC

DE

MAC BriarCol, Inc.

NY

MAC KGC-Mel, Corp.

NY

MAC LCBCS, LLC

DE

MAC LCBNA, LLC

DE

MAC SB, Limited

NY

Marlin Acquisition Corp.

FL

Trident University International, LLC

DE

University of St. Augustine Acquisition Corp.

DE

University of St. Augustine for Health Sciences, LLC

CA

University of St. Augustine Intermediate Corp.

DE

University of St. Augustine Parent Corp.

DE

Words of Wisdom, LLC

IL