Exhibit 21

ANI PHARMACUTICALS, INC.

The following is a list of subsidiaries of ANI Pharmaceuticals, Inc., omitting subsidiaries which, considered in the aggregate as a single subsidiary, would not constitute a significant subsidiary, as of December 31, 2021:

Name		Jurisdiction of Incorporation or Organization
ANIP Acquisition Company		Delaware
ANI Pharmaceuticals Canada Inc.		Canada
Novitium Pharma, LLC		Delaware
New Castle Pharma LLC		Delaware
Novitium Labs Private Limited		Delaware
New Castle Pharma Real Estate LLC		Delaware