Exhibit 10.1

LOAN AGREEMENT

This Loan Agreement (this “Agreement”) is entered into as of March 27, 2026 (the “Execution Date”) by and between:

Lender and Borrower are sometimes referred to herein individually as a “Party” and collectively as the “Parties.”

Section 1 Loan

Subject to the terms of this Agreement, the Lender agrees to make available to the Borrower a loan in the principal amount of NT$90,000,000 (the “Loan”).

The Loan shall be advanced to the Borrower on a business day designated by the Borrower with at least two (2) business days’ prior notice to Lender (the “Drawdown Date”).

On the Drawdown Date, the Lender shall disburse the Loan in immediately available funds by wire transfer to the following bank account designated by the Borrower (the “Borrower’s Account”):

Bank name: [___]
Branch: [___]
Account name: [___]
Account number: [___]

The Borrower may change the Borrower’s Account by providing written notice to the Lender prior to the applicable Drawdown Date.

Section 2 Interest

The Loan shall bear interest at a rate of 2.5% per annum, accruing daily based on a 365-day year.

Interest shall be payable in full on the Repayment Date (defined below).

If the Borrower fails to pay any amount due under this Agreement when due, interest shall accrue on the overdue amount (before and after judgment) at the rate set forth in Section 2.1 plus five percent (5.0%) per annum from the due date until paid in full.

Section 3 Repayment

The Loan shall be repaid in full on the date falling exactly one (1) year after the Drawdown Date (the “Repayment Date”).

On the Repayment Date, the Borrower shall pay to the Lender:

(a) the full outstanding principal amount of the Loan; and

(b) all accrued but unpaid interest.

Section 4 Representations and Warranties

Each Party represents and warrants that:

Section 5 Undertakings

The Borrower shall ensure that, unless otherwise agreed to in writing by the Lender, at all times any unsecured and unsubordinated claims of the Lender against the Borrower under this letter rank at least pari passu with the claims of all its other unsecured and unsubordinated creditors except those creditors whose claims are mandatorily preferred by laws of general application to companies.

The Borrower shall execute and deliver to the Lender the Promissory Note in the form set out in Exhibit A.

Section 6 Events of Default

Each of the following shall constitute an Event of Default under this Agreement:

The Borrower fails to pay any principal, interest, or other amount due under this Agreement when due, and such failure continues for five (5) business days after the due date (the “Grace Period”).

The Borrower becomes insolvent, admits in writing its inability to pay its debts as they fall due, makes an assignment for the benefit of creditors, or becomes the subject of any bankruptcy, reorganization, liquidation, or similar proceeding (whether voluntary or involuntary) that is not dismissed or stayed within thirty (30) days.

Any representation or warranty made or deemed made by the Borrower under this Agreement proves to have been materially untrue, incorrect, or misleading when made; provided that, if such misrepresentation is capable of remedy, no Event of Default shall occur unless the Borrower fails to cure the misrepresentation within the ten (10) days after notice from the Lender.

Upon the occurrence of any Event of Default and expiration of any applicable grace or cure period, the Lender may, by written notice to the Borrower, declare all outstanding principal, accrued interest, and any other amounts payable under this Agreement to be immediately due and payable, whereupon such amounts shall become immediately due and payable without further action.

Section 7 Miscellaneous

This Agreement shall be governed by and construed in accordance with the laws of the Republic of China.

No amendment shall be effective unless in writing and signed by both Parties.

The Borrower shall, upon demand, reimburse the Lender for all reasonable out-of-pocket costs and expenses (including reasonable attorneys’ fees) incurred in connection with the negotiation, execution, administration, amendment, enforcement or preservation of the Lender’s rights under this Agreement, including collection of the Loan.

All payments by the Borrower under this Agreement shall be made in full, in immediately available funds, without setoff, counterclaim, deduction or withholding (save as required by law). If any withholding is required by law, the Borrower shall increase the payment to ensure that the Lender receives the full amount it would have received absent such withholding, unless prohibited by applicable law.

No failure or delay by the Lender in exercising any right or remedy shall operate as a waiver. All rights and remedies of the Lender are cumulative and not exclusive. Time is of the essence with respect to all obligations of the Borrower under this Agreement.

This Agreement may be executed in counterparts.

[Remainder of Page Intentionally Left Blank; Signature Page Follows]

IN WITNESS WHEREOF, the Parties have executed this Agreement as of the date first above written.

Lender:

ASE Test, Inc.

Borrower:

Ainos, Inc.

EXHIBIT A

PROMISSORY NOTE

Amount: New Taiwan Dollars Ninety Million (NT$90,000,000)

Date of Issue: 27 March 2026

Payee: ASE Test, Inc. (the “Payee”).

Terms of the Instrument: Ainos, Inc. (the “Maker”) unconditionally promises to pay, in a single lump-sum payment on the Maturity Date, the Amount to the order of the Payee or such person as the Payee may designate.

Maturity Date: The date falling exactly one (1) year after the Drawdown Date specified in the Loan Agreement (the “Maturity Date”).

Place of Payment: Nanzi District, Kaohsiung City, Taiwan (Republic of China) (or such place as the Payee may designate).

Interest: From and including the Drawdown Date, interest shall accrue on the unpaid principal at 2.5% per annum, calculated based on actual days elapsed and a 365-day year, and shall be payable together with principal on the Maturity Date.

Overdue Interest / Default Charge: If the Maker fails to pay this Promissory Note on the Maturity Date, then in addition to the Interest above, overdue interest shall accrue on the overdue amount from the Maturity Date until paid in full at 7.5% per annum (i.e., 2.5% plus 5.0%), calculated based on actual days elapsed and a 365-day year.

Underlying Relationship: This Promissory Note is issued in connection with the loan obligations arising under the Loan Agreement dated 27 March 2026 between the Maker and the Payee.

Other Agreements: To the extent applicable, the Maker waives defenses relating to presentment, protest and other preservation procedures for this Promissory Note. The Maker further agrees that the Maker shall bear the Payee’s reasonable costs and expenses incurred in enforcing rights under this Promissory Note, including attorneys’ fees and litigation costs.

Maker: Ainos, Inc.

Address: 3050 Post Oak Blvd, Suite 510-T80, Houston, TX 77056